FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1966-lungLR-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1966-lungLR-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891062
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG10660.11963252837625216No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC9880.11087892873896542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1102.4465634E-432.9301159
TATACTG4400.029.6354395
TCAGGCG1606.861867E-527.1673459
TACAGCC5900.027.0138577
ATACAGC6450.026.9552336
CGAAAGC3800.026.6922253
TGCGGTC1100.009706045526.3440938
GCGAAAG4150.026.1883342
AGCGAAA3851.6370905E-1124.471991
GTAGCAT14100.024.1582471
TATAATA9900.023.4196012
GCTATAT2202.2344573E-523.0601481
CTACATC3655.7279976E-921.8343433
GGTTATA4006.9303496E-1021.7424241
GTATAAT3706.673872E-921.5465451
GTATTAG2104.323772E-420.707071
CATTCTA10900.020.6062932
GTCCTAT13400.020.552541
TCCTATG14350.020.1963442
TCGCTGA2959.621712E-619.646444
Produced by FastQC (version 0.11.5)