FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1966-lungLL-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1966-lungLL-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences897553
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC12120.1350338085884622No Hit
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG9620.10718030021625465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1209.576832E-636.2422141
GTGCGAA2600.036.226044
CCGGTAA850.002734972934.135086145
TGTGCGA3100.032.7239463
AAGGTCG1402.7598446E-531.049165
GTTATGT3700.029.3855781
GTTATAT2003.2614116E-728.9921552
TATACTG3001.8189894E-1128.9792185
GTATTAC3153.45608E-1127.6131151
GTGCGAT1050.007735044727.5992559
GATATAC1855.840151E-627.417363
ATTACCT7400.027.4143034
TTACGTG1606.839945E-527.1801452
TACTTAG3900.026.005547
ACCGTGC2259.162868E-725.7593028
GGTTATA2601.1468546E-725.0907651
GTACATA1500.00149602224.1614761
GTTGTCG1500.001500403424.1493479
CTGTGCG4002.7284841E-1123.5561262
GTAGTAT2502.2830354E-623.1950191
Produced by FastQC (version 0.11.5)