FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1915_d06_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1915_d06_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAAAAACTAAAAAACATACGAAATTTATTTTGTCTCTAAAGGGATTTC11.2345679012345678No Hit
GATCTGAACGGAAGAAGTCTGAGAGGAAGGATATTGAAGAGGCAGACACC11.2345679012345678No Hit
ACATTAAGCAATAGAAACTATATAAAAAAATCTGCCAAAAAATGGAAAGA11.2345679012345678No Hit
GGTTTATGAGATGCTTAACCGAATAGGCCACCCAGGCATCCCTCTTTTCT11.2345679012345678No Hit
GCTTCAAACAGGATTGATAGATTCTTTCTACCTTTTGTAGCAGTGCCTTT11.2345679012345678No Hit
ATAATGTCCAGCTTCTCTTTAAACCCTAAATGGCACCATATGGATTCTTC11.2345679012345678No Hit
GGATAGGAAAGGTCTGTCCTGACCACATCTGCGTTTCTATGTTCCTGTCT11.2345679012345678No Hit
GTTTTAATAATCTGCTAAAAACTGCATTTGTCTGGGCTGTTACTCCGTGC11.2345679012345678No Hit
GTAAACATTGTCACTATTTGTATGTTTAAATGGCAAACTAGAACTGTAGT11.2345679012345678No Hit
TNTANAGCCNCTNNGGGGNACNNNANNANANNNNNNTNNNNNNNNCNNNN11.2345679012345678No Hit
GAGTGGGAGGCAGAGGGAGAAGCAGGCTCCATGATGAGCAGGGAGCCCAA11.2345679012345678No Hit
ATGCTACATTTCGCTGTGAACCTAAAATTGCTTTAAAAAAAAAAAGTCTA11.2345679012345678No Hit
ACATTAATCTGCCCCAAAAAAGAAAAATAATAAGAAGCAGGCTCAATTTA11.2345679012345678No Hit
GTTATGGTGTCTTCCAAAACAGACTTGCTAATTACACAATTGTGTGCTAT11.2345679012345678No Hit
AGCGAAAGCAGGCCTGAGAAATAAATCTATACCCCTAAATGCATACTTTA11.2345679012345678No Hit
ACACACATTCTTTTCAAGTGCACATAAGACACTCTCCAGAAGAGATAACA11.2345679012345678No Hit
GGGTGGGGGAGGCGGCAGAGTGCGCCTGACCCTGCCTCAGACCCAGCGCT11.2345679012345678No Hit
AGTAAAATAAGAACTCAAAATACTACATAACGAAATCTGAAAAAACAAGA11.2345679012345678No Hit
GGACTCCATCCCCAGGACCCTGGCATCATGACCTGAGCCAAAGGCAGAGG11.2345679012345678No Hit
GAGCAGGGAGCCCAATGCAGGGCTCGATCCCAGGACCTTGGGTTTATGAG11.2345679012345678No Hit
GAGTGGGAGAGGAAGAAGCAGGCTTTCCGCTGAGCAGGGAGCCTGATACA11.2345679012345678No Hit
ACTTATTGCTGGGCTTTTGGAGTGTAGTGTTATCACCATGCCAACGTGTC11.2345679012345678No Hit
TGTATGTACACATATCCAAGTAATCCATTTTAAAAGGGCTAGATGAATTC11.2345679012345678No Hit
ACCTAGGCGCCCCTCAGGTGGGGCATTTTTAATTTGAGCTAAAAATACTT11.2345679012345678No Hit
CTAGCATAGTCCCCTCCTAGCTGAGGTTATCCTGTATTGGATAGTGAAAT11.2345679012345678No Hit
GGGTGGCTCAGCTGGTTAAGCCACCGCCTTTGGCTCAGGTCATGATCCCC11.2345679012345678No Hit
ATTTTTAGTAGCATGTTATTTAACATTCTTGTATTTATGCTCTTTCCAGA11.2345679012345678No Hit
AAATGCATACTTTAGAAAAGAGGAGGGATGCCTGTCTGGCTTAGTTGGTA11.2345679012345678No Hit
GTTAGGAGGCAATTACAATAACCCAGGCATGAGAAGATGGTATCTCACTC11.2345679012345678No Hit
GGATTTAACCACAGGGAAATTTTTGGGAAGACCACATATACATGGAAGTT11.2345679012345678No Hit
GAGTCCAGCAGATAGCGTTTTACCCAGAGATGCTAGTAACATTACCTGTT11.2345679012345678No Hit
CTGTTACTCCGTGCTGTTCACTTCTAGGCGTCAAAGCAAATCTGTCTGAA11.2345679012345678No Hit
GTGCTACAGAAGAGAGAGAGAGATAGAAAATAGCAGTTTTTCTCTTCATA11.2345679012345678No Hit
ATAAAAGACAGGAGTTTTGTTTTGTTTCGTTTTTTTAAAATAGTTTTTCA11.2345679012345678No Hit
ACAAATTATATTGCCTTACACCCAGGAAACTGCAGGGGAAAAATTTGATA11.2345679012345678No Hit
AACAAGGCCTTGACCCTATATCCAGAGGTTGACAAATTATAGATACATTT11.2345679012345678No Hit
GGGGTGTCTCCTGCCAAGGCGTGAGAAAATAATTACCTGGACTTTTCTGG11.2345679012345678No Hit
GGGTCATAGAGCATCAAAACACTCCAGTGTCTTACATCTGAAATGAATGT11.2345679012345678No Hit
ATACCCCTAAATGCATACTTTAGAAAAGAGGAGGGATGCCTGTCTGGCTT11.2345679012345678No Hit
GAGACACCCAGATGTCCTCATCCTCTCTAAACTGGAGCCAGCAACGTCTT11.2345679012345678No Hit
ATGCAACCTGGTTTACCTCTTAATTTTCGTCCTGTGAAACCTTGAGCAGA11.2345679012345678No Hit
GTTTCTGCTGCATGACTATCTGTTATAGAATAAAAATTTGGAAGAACCAT11.2345679012345678No Hit
GTGTTCTAGGATCGCTCTTCTTAAGATTTATGGCTTTTGAGGAAGAGAAT11.2345679012345678No Hit
TTGGAGAAGTTATGGTGTCTTCCAAAACAGACTTGCTAATTACACAATTG11.2345679012345678No Hit
CGGTGAGCTGGGGGCCCAAAGCAGGGCTCCATCCCGGGACCCTGAGATTG11.2345679012345678No Hit
GTGTTTAATGATTACTTGGGTCACATGTTCACAAGGATGACCTCCATCTA11.2345679012345678No Hit
ACCTTAAGCCAGCAGATGTAGCAGGAAATTCTCCAGCTCATAAAGTGGAG11.2345679012345678No Hit
CTTGTATAAAATACTACTTTTAATGTCAGATTCCTTTAGTGAGTCTAACC11.2345679012345678No Hit
GAGTGACATCTCTGTTCAAGTAAGCAGACAGGCTGAGACACCCAGATGTC11.2345679012345678No Hit
ATAGAAAATAGCAGTTTTTCTCTTCATAATCATATGAATTATTCACAGAA11.2345679012345678No Hit
GGCAACTCCTAGGTGATTCCTAGTAGAAACATGGCTACATGTTCTTTACA11.2345679012345678No Hit
GCAGGGGACGTGGGAGAGGGAGAAGCAGGCTTCCCGCTGAGCAGGGAGCC11.2345679012345678No Hit
GGCAGAGGCAGAGGCAGAGGAAGCAGCCTCTCCTTCGAGCAGGGAACCTG11.2345679012345678No Hit
GATAAAGGGCTAGTATCCAAAATCTATAAAGAACTTAAGGGGTGCCTGCG11.2345679012345678No Hit
GACTTAGCCCCTGGCAGGGGGAATGAAAAAAGAAAAAACTCTTAAACTTG11.2345679012345678No Hit
GAGTATGACATCATGGTCAATATCACTGAGAAACCAAAGGAGAGAAGTGA11.2345679012345678No Hit
CAACTGAGCCATCCAGATGTCCTTTAAATTATATTTTTAAGTTCTCAGAA11.2345679012345678No Hit
GGATATGTTGTTTCACTGAATATTGATGTCAAAATGAAGCAAGATTTTAA11.2345679012345678No Hit
TTCCAGGTCTGCCACACCACAAGCTGCTTTGACAGAAGTCAAATTATATT11.2345679012345678No Hit
TATCTATTATTTCCACTTACTTTGAATTTATTTTACTCTTCTTTTTTTTA11.2345679012345678No Hit
GTGGGGGGAAAAAAATTTTTTTTTTTAGACCCAGGGGGGGGGAGAAAAAA11.2345679012345678No Hit
GCCTGACGCAGGACTCAATCTCAGGACCCTGAGACCATGACCCGAGCAGA11.2345679012345678No Hit
AATTAGAAGATTATATAAATCTTAATTCATCTAATTCACTCTTTGCTTTT11.2345679012345678No Hit
GCAGGGCAGGTCATGGCCCCTTTAACTGGCTGATCTACAGCTTAAGATAG11.2345679012345678No Hit
CGGAACCTCGGTCCCACAGACACTTAGGCAGCTGACTCTACATTCTGGCT11.2345679012345678No Hit
GAGCAGAGAGACCGACATGGACTCGATCCCAGGACCCCGAGATCCAGACC11.2345679012345678No Hit
GGGAGAGCCGATGCGTTCAAATTCCACACATGCAAAATAAACGAACGAAG11.2345679012345678No Hit
AATCAACTGAGCCACCCAGGCGCCCCTGACCTTTTATTTTTAAGGAAATG11.2345679012345678No Hit
GTAGAAATTCTGAGAAGGGAGAGCCATGGGCAGTTGTGGGCAGACTTCCT11.2345679012345678No Hit
ATATAGATGCAAATAAACCAAATTAGCTTAGAAGCACGTTCTTCCCCAGT11.2345679012345678No Hit
GGCATCCCTCTTTTCTGTTTTCCATTACTACTTTCTTTTTTCTGTCTTCA11.2345679012345678No Hit
GAGTAGGTGAGGGAGAATCAGACTTCCTGATGAGCAAAGAGCCTGATTCA11.2345679012345678No Hit
AGCGAAAGCAGGTGCAAAAGCAGGCTGGGTAAAGGCTCTGGGTGAAATGG11.2345679012345678No Hit
AAACAAGGCGTATAAATTTACTAAGAACATCAAGACCTCTAAGTGAACAA11.2345679012345678No Hit
GTGTAGTGGGAGCAAAACTAGGACAAAAAATTCTTAATCTGGAGATTGAA11.2345679012345678No Hit
ATTTAAAAATGTCTAAAGGCTGAAGCTACAGGTGTATAACTAGAGACAGG11.2345679012345678No Hit
TCATTCAGCCGTTAACCCCCTTTTATGACGAGAAGGTGAGGCCGCCTCTA11.2345679012345678No Hit
GAGAGAGAAGAGTTAGCAGCTTCCTGCTGAGCTGAGAGCCTGATGCAGGG11.2345679012345678No Hit
GATATCTTGAGGACAAGATGGTTTTCCTTAAGGCTGCTCATTCACAAAGC11.2345679012345678No Hit
CCTGTCTGGCTTAGTTGGTAGAGAATATGATTCCTGATCTCAGGGTCTTG11.2345679012345678No Hit
TATTTAGTCTCCCACGATTCCTGAGAGAACAGTCTTACCACATTCTCATA11.2345679012345678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTA50.0145.09
CAACCTG50.0145.04
TGCAACC50.0145.02
AACCTGG50.0145.05
GCAACCT50.0145.03
CTGGTTT50.0145.08
ACCTGGT50.0145.06
ATGCAAC50.0145.01
GTTACAC50.0145.0145
CCTGGTT50.0145.07
Produced by FastQC (version 0.11.5)