FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1915-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1915-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529231
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTAACACAGTGCGGCCTCCCTACAGGGCTTCATGGCCACAGTAACCA11980.22636618036358416No Hit
CACCAGGGATGCTCCAATAGGACTCAACACATCTGGAAGTAATCCAAAAG5480.1035464664768315No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC2050.045.941753
TGCTAAC4350.043.309612
GCTAACA5050.035.864653
GCGAAAG2650.035.546572
TATACTG4550.035.0258145
GTGCTAA5900.034.394451
CCTAAAC2353.45608E-1133.9111753
AGCGAAA3000.031.405411
GTATACT2601.10958354E-1030.6504864
CATAGCG1204.075287E-430.1832267
GTATAGC1000.0060825428.9896091
CTAACAC6700.028.1136054
GTATTAG1555.5090204E-528.0544591
TGTATAC3102.910383E-1128.0438443
TATCCTG4150.027.9285765
TATAGTG1606.847624E-527.1726172
ATACTGT5900.027.0114336
GTCTAGG5150.026.737991
TAGGGGG4950.026.3442154
GTCCTAT3251.4879333E-924.5296691
Produced by FastQC (version 0.11.5)