FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1915-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1915-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821716
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC9060.11025707178635928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1550.056.124671
TTACGTG1953.6379788E-1240.8892632
TACGTAC900.003637914832.2040063
CGTGTTG2651.4006218E-1030.0718255
TACGTGT2802.6375346E-1028.466043
CTAATAC2553.0395313E-928.4152983
TGTACCG1050.007735859627.5983835
AGCGAAA2954.820322E-1027.0317961
ACGTGTT3108.6401997E-1025.7096964
TATACTG3709.094947E-1225.4539155
GTTGACA7700.025.4201011
GCGAAAG3151.035005E-925.3124032
GGTTATA3551.546141E-1024.5051361
GTATTAG3001.7074854E-824.1647851
GTAATAT2701.6493505E-724.1647851
CAGGTCG4550.023.8875857
GTTATGT5900.023.3456421
GGTCCTA11250.021.9013949
TACACCG2003.1181154E-421.7337285
CTACTGA10700.021.6673436
Produced by FastQC (version 0.11.5)