FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1915-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1915-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891134
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA851.31312845E-859.660118
TATCGAA1004.7288268E-850.7139439
GCGTAGA450.00964873248.3207171
GGACCGT3150.045.9912876
CGTAGGC1602.0008883E-1145.34657145
CCGTGCG3250.042.3545049
TGTATCG1355.00273E-737.561667
AGCGAAA2800.036.240541
AAGGTCG1001.3985405E-436.2181436
GACCGTG4400.034.5738037
ACCGTGT3650.033.7412348
ACCGTGC5700.031.7738698
GCGAAAG3100.030.385042
GTATAGG1703.0173505E-629.845151
GCACCGT5050.028.6876356
CCGCACG1306.453951E-427.90558145
TACCCCG1306.5200793E-427.8569765
GTCCTAC3153.45608E-1127.611841
AGGACCG6050.027.5346645
AGCACCG5900.027.0071035
Produced by FastQC (version 0.11.5)