FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1915-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1915-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006418
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4450.042.338261
GCGGTAT1553.4964614E-837.3875246
GCGAAAG5050.037.3005452
CGGTATC1751.01632395E-733.1163147
CGAAAGC6250.031.2949183
TATAGCG2003.2744174E-728.9796562
TATACCG2003.278692E-728.9753345
TAGCGGT2003.280129E-728.9738924
AGCGGTA2456.491791E-826.615
GTATATA5400.025.4964391
GTATAAG3501.3096724E-1024.844651
CCGTCGC1500.001489563824.179781145
TACTCCG1550.00181747123.3672035
GTATAGG3753.092282E-1023.1883371
TACATCG2852.8017166E-722.8752635
ACATCGT3554.1654857E-922.445686
GTATAGA9400.022.3557781
GTCGGTA2303.1289084E-522.07719145
ATACCGA2303.162751E-522.046456
TATACTG12250.021.8793325
Produced by FastQC (version 0.11.5)