FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1914-d12-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1914-d12-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579351
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC10840.18710591679310123No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA5940.10252851898072152No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT5840.10080244963761174No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCGC502.3074354E-672.487171
TATACTG2550.036.9287155
AGCGAAA2401.8189894E-1236.2435841
TTGACCG800.002044601436.2185485
CGAAAGC2401.8189894E-1236.2185443
GCGAAAG2807.2759576E-1231.0525112
GTTATGT3450.029.4150811
GTACAAT2853.237801E-1027.97751
TCTATAC10000.025.352983
GTATTAC1500.001495054524.1623881
GTATTAT3952.3646862E-1123.8565371
CTTATAT1550.001809434723.3829571
GTTCTAT9700.023.1660021
CTATATA2252.6615427E-522.5418242
GGATATA1952.6236882E-422.2941112
TATTATA1952.6236882E-422.2941112
CTACTGA5850.022.2883366
TACTGAA6200.022.200387
CTATACC11100.022.1879394
AATTAGT2303.160182E-522.0460725
Produced by FastQC (version 0.11.5)