FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1914-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1914-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871266
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCCG450.00961339448.365524145
GTGATCG1105.295673E-639.5103959
TATACTG4050.037.5484775
GTATTAG1406.633418E-736.2324371
CAGTCGC800.002045354836.217869
GCGAAAG4750.033.5549622
AGCGAAA4800.033.2130661
CCGACTA1551.4768866E-632.7072684
GTAATAT3650.031.7654271
GAGCGAA2755.456968E-1231.6210371
CGTATAC1153.1793248E-431.4919833
CGAAAGC5150.030.9416393
TATGACG950.00474884730.49753
ACTACGC1453.50144E-529.9854641
CCACCGA2003.2673415E-728.9859491
CTACGCA1504.4195403E-528.9792862
GTTCGAT1100.00971280626.3402619
ATACTGT5400.025.4822526
GTAACGG1751.2631257E-424.8351089
GTATATG3952.3646862E-1123.84921
Produced by FastQC (version 0.11.5)