FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1914-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1914-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771216
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT451.1987844E-464.3889546
GCGTAAA450.00965382348.3136521
TCGTCGA700.001060300441.398279
AGCGAAA3100.032.7286031
ATCGTCG900.003640740232.1986548
GCGAAAG3250.031.2119752
CGTTGTA1000.00609535728.9787867
ACATCGT1255.178802E-428.9750296
GCACCGT3151.0440999E-925.295666
CGAAAGC4101.8189894E-1224.7347833
GTCCTAT7300.023.8259091
AGCACCG3452.9922376E-923.0960395
GTCTTAG5700.022.8854141
GTTATAC2252.6682208E-522.5361353
CTTAGAC4058.1308826E-1021.4629843
GCGTGCG1700.003105308921.3079349
TACATCG1750.003676284820.696455
GTATATA6100.020.196691
TATACAG3951.4246325E-820.172495
ATAAGAC3301.1467182E-619.7557013
Produced by FastQC (version 0.11.5)