FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1914-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1914-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907887
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGC1000.006098356628.9764122
AGCGAAA4600.026.7775941
GCGAAAG5300.024.6080441
CGAAAGC5150.023.9099353
CTACGTG1600.002183202422.641579
GGTTATA2252.6633617E-522.5421831
GCACCGT4256.184564E-1122.155996
GTCTAAC2506.0010294E-520.2879641
GTATTAT8700.019.9881421
AACCGTG2556.991318E-519.8890657
GTATTAG3651.3683166E-719.8512381
GTTATGT6300.019.5518951
CTAATAC3001.1141854E-519.3154773
CATAGCA8650.019.2617782
GTATATA5305.820766E-1119.139591
GAATCGC1900.0059189819.0613276
ATAGCAC6550.018.7994163
CCTACAC4253.3056494E-818.7473753
GTATAAA8350.018.2227251
CACCGTG6052.0008883E-1117.963567
Produced by FastQC (version 0.11.5)