FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1913-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1913-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784474
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC25390.32365636082266586No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG21920.2794228999303992No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC21730.2770008948671339No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG21290.27139204103641423No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC20790.2650183435015055No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG20680.26361613004382556No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG18030.2298355331088092No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC17390.22167720026412602No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCAGACCATTGACAAGG17180.21900024729946432No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA16410.20918475309570492No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG16010.2040857950677779No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT15550.19822199333566184No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT14780.1884064991319024No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA13850.17655142171697213No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT13050.16635350566111815No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT12810.16329413084436195No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA12140.15475337614758425No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT11810.15054673577454447No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC11620.14812473071127916No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG11350.1446829340424284No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA11130.1418785071270686No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG10830.13805428860612334No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT10620.13537733564146168No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC10560.13461249193727262No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA10140.1292585860079493No Hit
CTATAGGACTTGCAAGCTAGTTGGAATCAACATGAGCAAAAAGAAGTCTT10120.1290036381065529No Hit
GCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCTAG9850.12556184143770222No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC8870.11306939426928106No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA8830.11255949846648837No Hit
GCCATTCCACAATATCAGTAAATATGCATTTGGAACCTGCCCCAAATATG8720.11115728500880845No Hit
GTGTAACCCTCTGAACCCGTTTGTCAGTCATAAGGAAGTTGAATCCGTCA8710.11102981105811027No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA8650.11026496735392123No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG8590.10950012364973216No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC8570.10924517574833582No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA8560.10911770179763765No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC8520.10860780599484494No Hit
ATCATATTCCATGCTCTTGGCCGGACCATGGGCTGGCATTACCACAGCAT8500.1083528580934486No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT8420.10733306648786321No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA8210.10465611352320153No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG8020.10223410845993622No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGAA100.0071087605144.87589
AGTAGAA3250.0111.528341
CACACTG203.8789416E-4108.6568459
GCAGGTA2800.0106.1171952
GGTACTG2800.0103.44975
GACTTAA259.360805E-487.05884145
TTGCACG259.360805E-487.05884145
CTATAAC750.086.897755
TATAACT900.080.4711466
TATGGCG300.001930400472.54903145
GTAGATG406.675834E-572.4702762
GTACTGA4750.071.6616746
AGGTACT4500.065.9863364
AGCATAC554.0445175E-665.95366145
TCTATAA1009.094947E-1265.181634
CAGGTAC4650.062.3081973
ATATCAC1200.060.3610653
AGAAACA6200.058.4064754
GACAGTA4600.055.1157957
TGCAACC400.00603423754.41177145
Produced by FastQC (version 0.11.5)