FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1913-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1913-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590322
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA21090.1326146528816177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTA2700.040.30136145
GCGTTAC750.001485588138.6563871
GTATAGG6700.037.863071
TATACCG2900.037.4640665
GTTACGT2800.036.2403681
TACCGAG3100.035.0525447
GTGCGAT1957.054041E-933.433689
ATACCGA3550.030.6044486
GCGATTA950.00471460630.544188145
TATACTG8200.030.0321715
TATGTCG1050.00774594727.5925835
TGCACCG2705.6261342E-926.8261185
CACCTAA19250.026.72812
CGTGTTG4100.026.4989745
TCGATTA6850.026.475346145
CCTATAC7550.025.9080163
GCGAAAG7650.025.5814361
CGAAAGC7850.024.9249692
GTCGCAT1751.2511971E-424.871696145
CTATACT8800.024.693784
Produced by FastQC (version 0.11.5)