FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1913-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1913-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842330
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC10340.12275473982880818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3900.042.7452051
ATCGTGC1458.762836E-734.965888
GCGAAAG5050.034.4402772
GGTACTG5400.033.536085
GTACTGA5850.033.4309046
ACACCGC1551.4751568E-632.711966
GCAGGTA6200.031.5586782
ACTGATC6550.030.9621548
CGAAAGC5650.030.7755833
ATACACG1453.505934E-529.9796623
GTATAGG2951.4551915E-1129.4837991
CAGGTAC6700.029.1966113
TGTATCG1255.179049E-428.9751765
TAAGCGT1358.124992E-426.8272696
GTCTAAC1907.1682407E-626.7035271
CTATAGT1958.770148E-626.018821
TACTGAT8150.025.77247
GTAATAC2701.6590639E-724.1502823
CAATTCG4201.8189894E-1224.1445459
AGGTACT8150.024.0021234
Produced by FastQC (version 0.11.5)