FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1912_d08_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1912_d08_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4863
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCAGGGATGCTCCAATAGGACTCAACACATCTGGAAGTAATCCAAAAG100.20563438206868187No Hit
GTGCTAACACAGTGCGGCCTCCCTACAGGGCTTCATGGCCACAGTAACCA80.16450750565494549No Hit
CAGTAACCATGAGAAAGGAGACATTTCGCCCATAATCCACACACAAGATT60.12338062924120913No Hit
TGCTAACACAGTGCGGCCTCCCTACAGGGCTTCATGGCCACAGTAACCAT50.10281719103434094No Hit
GGTCTGGCCCTCTGCAACCCCCCTGCACCTCCCTGCAAATCTGCTGCTTT50.10281719103434094No Hit
CTCCAATAGGACTCAACACATCTGGAAGTAATCCAAAAGACAAGCCCTCC50.10281719103434094No Hit
GTTGTATACTGTGGGTCATAATTCAACACTGTATTATTCTGGTGTTACTG50.10281719103434094No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTAT100.00687628144.999981
TACAGTG100.00687628144.999984
CAGGGGG100.00687628144.999984
GGGGGGG1552.9334825E-49.354839145
Produced by FastQC (version 0.11.5)