FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1912-d12-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1912-d12-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713141
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG1157.1557733E-637.813789
AGCGAAA3250.035.6882131
CGAAAGC3250.035.6781923
GCGAAAG3400.034.1113362
CTATACT1957.017661E-933.4459574
ATATAGG2008.976713E-932.61213
GTTACGT900.003629574632.2185251
CCTATAT1409.998453E-425.8898851
TATACTG2601.1497286E-725.0827085
GTACCTA3851.6370905E-1124.4674036
GTGTAGG3803.6379788E-1022.8921091
GTATATA4205.2750693E-1122.4379021
GTATTAG3253.990681E-822.3051321
ATCTAAT3055.341608E-721.390991
TAGTACC4801.4551915E-1121.1359864
GGTCTAG1750.003660145220.7119081
TAGCATG8750.020.7104552
TACGTGT1750.003666106420.706093
TAAGGCT3851.05555955E-820.704644
TCTAATA3901.2205419E-820.4449352
Produced by FastQC (version 0.11.5)