FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1912-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1912-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864231
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT10490.12137958485636365No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT8990.1040231141905347No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG8690.10055182005736892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3500.051.758071
GCGAAAG3750.048.2963372
CTATACG604.966247E-448.2907378
TATACTG7500.036.6988335
TATACGA850.002753654734.0875789
GGTCGTT2800.033.631057
AAGGTCG2850.033.0391165
CGAAAGC5600.032.337553
ATAACCG950.004748713730.4976465
GTCGTTT3201.8189894E-1229.427178
ACGGTTC1255.17992E-428.9744459
GTGATCG1306.515725E-427.860049
AGGTCGT3403.6379788E-1227.6945536
ATCGAAT1855.77884E-627.454397145
ATACACG1100.00971525326.3388776
AATATAC7100.025.5056723
GTATATA6400.024.9085731
TTATACT6900.024.145374
TACTGTG7700.023.5182197
GTCTATA1851.837017E-423.5009631
Produced by FastQC (version 0.11.5)