FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1912-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1912-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121865
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAGAAAAGAGGAGGGATGCCTGTCTGGCTTAGTTGGTAGAGAATATG4780.392237311779428No Hit
AAAGCAGGGAGTGGGAGGCAGAGGGAGAAGCAGGCTCCATGATGAGCAGG2830.2322241824970254No Hit
CCCCTAAATGCATACTTTAGAAAAGAGGAGGGATGCCTGTCTGGCTTAGT2610.2141714191933697No Hit
GCCTTACACCCAGGAAACTGCAGGGGAAAAATTTGATAGTCCAAAAAGTA2490.2043244573913757No Hit
GGCCTGAGAAATAAATCTATACCCCTAAATGCATACTTTAGAAAAGAGGA2110.17314241168506134No Hit
GAGTGGGAGGCAGAGGGAGAAGCAGGCTCCATGATGAGCAGGGAGCCCAA1970.16165428958273498No Hit
GCATTACATCCAGTGAACAAATTATATTGCCTTACACCCAGGAAACTGCA1910.15673080868173797No Hit
GAATATGATTCCTGATCTCAGGGTCTTGAGTTCAAGCCCTACCCCGGGGC1890.15508964838140565No Hit
GTGGGAGGCAGAGGGAGAAGCAGGCTCCATGATGAGCAGGGAGCCCAATG1840.15098674763057482No Hit
TCCCAGGACCTTGGGTTTATGAGATGCTTAACCGAATAGGCCACCCAGGC1750.14360152627907932No Hit
GGTGGAGGGGCAGAGGGAGAGGGACAAGCAGACCCCCCGGTGAGCTGGGG1740.14278094612891315No Hit
ACATATAGTGCCAATTTGGATCAAAAGTCTTTAATCTCTTTCTCTCCTAA1630.13375456447708528No Hit
AGCAGGGAGTGGGAGGCAGAGGGAGAAGCAGGCTCCATGATGAGCAGGGA1620.13293398432691914No Hit
AAGCAGGGGAGTGAGAAAAGTACATGTTTTGTTTTAATAATCTGCTAAAA1610.13211340417675296No Hit
GAAATAAATCTATACCCCTAAATGCATACTTTAGAAAAGAGGAGGGATGC1600.13129282402658682No Hit
ATCATGACCTGAGCCGAAGACAGACGCTTAATGACTGAGTCACCCAGGTG1460.11980470192426045No Hit
AGTAGAAACAAGGCGTATAAATTTACTAAGAACATCAAGACCTCTAAGTG1410.11570180117342961No Hit
AAGCAGGCCTGAGAAATAAATCTATACCCCTAAATGCATACTTTAGAAAA1360.11159890042259878No Hit
GCCCAATGCAGGGCTCGATCCCAGGACCTTGGGTTTATGAGATGCTTAAC1330.10913715997210027No Hit
GGATAACTCAGCTTCAAACAGGATTGATAGATTCTTTCTACCTTTTGTAG1330.10913715997210027No Hit
AAATAAATCTATACCCCTAAATGCATACTTTAGAAAAGAGGAGGGATGCC1320.1083165798219341No Hit
AAATGCATACTTTAGAAAAGAGGAGGGATGCCTGTCTGGCTTAGTTGGTA1310.10749599967176794No Hit
AAACAAGGAAGGGCATTCCAGGTCTGCCACACCACAAGCTGCTTTGACAG1290.10585483937143561No Hit
GTAGAAACAAGGCCCATTTCATATGGAGAAGTAAAAATGAGTCAGCAAGT1270.10421367907110327No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAGC100.007117125144.768973
AGCGAAA1600.0113.193661
GAGCGAA300.001937583972.443941
GCGAAAG2750.065.858132
GTACACT350.003581546262.0438464
GGATAAC606.8342197E-660.3204043
CGAAAGC3250.055.6803743
GTATTGA400.00606653854.310659
GTACTGC400.006076401654.2883646
TACTATG450.00964841148.295962
TTCCCGC450.00966408348.2761349
ACTGGGG450.00966408348.2761348
ATCCTAT450.00967977448.2563256
CAGGGGT752.5613357E-548.2563254
GTAGAAA3200.047.5413361
AGTAGAA1402.7102942E-1046.5711061
GTTGTAC951.9171057E-645.7540661
AGGACAG803.750409E-545.2403035
AAGGCAC657.368798E-444.54435
CAAGGCG855.3510594E-542.596599
Produced by FastQC (version 0.11.5)