FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1912-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1912-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771508
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA9750.12637587685416096No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT9300.1205431440762766No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAC350.003573040562.1127661
CGAATTA350.003576719262.0966347
GTTACGT1004.719368E-850.725431
CCGTACA604.959365E-448.3036582
GTGATCG855.3638378E-542.615349
TTAGGTA1905.462425E-934.3165664
TATACTG5400.033.5376745
GTATATC1957.021299E-933.4453351
ACACCTA8800.031.291661
GTTATGT4300.030.3341431
TATATCC2202.2762833E-829.640882
CACCTAA9950.028.399642
GACGACA1306.509842E-427.8638769
CGAAAGC2654.5838533E-927.3416942
GCGAAAG2705.5879354E-926.8388521
ACATGGT6850.026.4384565
GTAGAAA5850.026.009662
GTATACT1400.001003597625.8735984
ACGTGTT2259.162213E-725.7586064
CAGGTCG4001.8189894E-1225.3561277
Produced by FastQC (version 0.11.5)