FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1911-d12-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1911-d12-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663250
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGAC100.007114259144.836674
AGCGAAA2101.4446414E-831.0575141
GCGAAAG2451.9826984E-929.5718882
GTATATA4950.027.8158231
TAGGCCG1100.00972611726.3319535
TAGTATC1751.2647422E-424.8291424
GTATAAG3352.1173037E-923.7953111
GATGTAC2151.8797386E-523.579848
CGAAAGC3102.4398105E-823.3642793
TATAGGT2502.2997065E-623.1738664
GTGTAAG4205.2750693E-1122.4304281
GTTATAG2303.16371E-522.0436883
GTCTTAG3457.530252E-821.0050851
TAATACT3901.2334567E-820.4256844
GTAATAT2155.0689385E-420.22351
ATAGTGC1800.004339426320.111656
CATATAC2608.132834E-519.5001853
ATACTGT4904.2018655E-1019.2086816
TATATAG4553.4560799E-919.107992
GTCCTAT7300.018.8614141
Produced by FastQC (version 0.11.5)