FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1911-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1911-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712882
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACGC450.0096653348.2987063
AGCGAAA3050.040.3950731
GCGAAAG3400.036.236751
CGAAAGC3550.034.698312
TAAGCGC1501.1396478E-633.8090936
AAGCGCT1102.444708E-432.9332477
ACGGTAG900.003641539732.1968775
GTCCGGG1153.1679714E-431.5102211
TCAGGCG1000.006092508328.9812589
CCGACTA1100.00970358926.3447484
GTACCTA3201.2496457E-924.9040186
ATATAGG1851.8344827E-423.5049231
TTAAGCG1851.8397317E-423.4950165
TTAGACT3604.8821676E-922.1369084
GTATATG5151.8189894E-1221.1013763
GTGTAGC2455.1257488E-520.7067181
TATAGGG2104.3292498E-420.7023532
TAGTACC3601.1837801E-720.1244624
GTCTAGA2908.270075E-619.9926931
CTTAGAC2205.929629E-419.758563
Produced by FastQC (version 0.11.5)