FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1911-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1911-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845887
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG13730.16231482455694438No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT12550.14836497073486174No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA10080.11916485298863796No Hit
GTCTTCCGACTGGGCCTTTCGCTTAATTTGATGCCTGGCAGTTCCCTACT9780.11561827998302374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG4100.045.9429449
AGCGAAA3800.041.9562761
AGTGATC5600.033.63687
TATCGCT1352.151375E-532.2012335
CGAAAGC5100.031.255993
GACGGAT3300.030.7357237
GTTACGT1204.067392E-430.1958051
GTGATCC6100.029.6920018
GTCATAG6150.029.4575792
CATAGTG6300.028.75114
GCATCGA2054.0149644E-728.311111145
GCGAAAG5850.027.2520352
GGACGGA3306.184564E-1126.3464646
ACTGGTC5100.025.5700558
TAGCGTC1500.001500278224.1494987
ATAGTGA8400.023.2883915
TTATCGC2202.2395277E-523.0531564
TCATAGT8200.022.9741923
TAGTGAT7900.022.9280936
GGTCATA10250.022.624761
Produced by FastQC (version 0.11.5)