FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1911-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1911-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666900
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCG100.0071056327144.895516
ATCGGAC400.006067281654.335823
TATCGGA553.2261093E-452.7011382
TTGTACG750.001488293838.63884
GTATCGG850.002745982734.1058541
GCGACTG850.00274800434.1007359
TAAACCG950.004742870630.5043165
ATCGATA1050.00772530627.6053568
GATCCGG1358.103301E-426.8385439
GATCGAT1600.002180668522.645027
AGCGAAA2903.2936077E-722.4922221
GTACTGT2755.2389696E-621.0757126
CGAAAGC3457.552262E-820.999353
CTACACT3457.552262E-820.999354
TATACTG4251.4806574E-920.4558375
CCACTAT2155.065496E-420.2255631
TAGTACT2205.929252E-419.7584784
TAGTAGT3051.28184565E-519.002694
GTATATG3451.7460006E-618.9065061
CTATAGA2701.08134685E-418.7897991
Produced by FastQC (version 0.11.5)