FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1911-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1911-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513730
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGT300.001939428972.460157
GCGAAAG1000.006082276328.9897081
CTAATAC2252.8265276E-828.9840583
TTATACT5750.028.9812364
TATACTA3550.028.5702655
TCTAACC2001.068699E-525.3610523
GTCCTAT5200.023.693511
TCCTATG5900.022.1086392
GCAATCC1700.003100638721.3118088
CGCGAGA707.8118814E-420.7069341
CGCACTC707.8292016E-420.6988665
TAATACT3551.0192525E-720.409324
GTATTAG2857.108669E-620.3436551
GTAATAC2155.070284E-420.2214373
ATACTAC2908.289822E-619.9851136
TATAGTG4351.9754225E-919.9851115
TACTACC2556.978488E-519.891027
GTATATA5900.019.654041
GTTATAC2608.101693E-519.5085013
TACACTA2608.113834E-519.50475
Produced by FastQC (version 0.11.5)