FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1910-d12-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1910-d12-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991145
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACG400.00607028354.3309864
TAGACCG604.964844E-448.294214
ACGACGA450.00966999448.294215
GCGAAAG2750.042.162582
GTGATCG1209.603684E-636.2279749
GTACGAC850.002752198134.091753
AGCGAAA3900.029.7375341
GTCCTAC5000.027.5443941
CGAAAGC4400.026.3436263
GTCTACC2051.2922832E-524.751061
GCACCGT3551.5643309E-1024.4884416
AGCACCG3551.5643309E-1024.4872045
TACACCG1801.5286451E-424.1471045
CCTACAC5550.022.1903533
TATACTA2653.8064918E-621.8690745
TCCTACA7550.021.1161922
CCGTTCA1750.00367161120.70179
GTTACGT2155.063026E-420.2284411
CTACACT6450.019.093064
GTATTAC3502.0029256E-618.6390631
Produced by FastQC (version 0.11.5)