FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1910-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1910-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615659
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG6330.10281665662322811No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC6310.10249180146802044No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG752.559753E-548.3002979
CTAGTAC1105.2828036E-639.521643
TATACTG3100.037.3968245
GTTACGT900.003629185332.2185361
AGTCCGC1402.7364447E-531.085571145
GCGAAAG2003.2541539E-728.9966831
GGTTAAC1000.006076056528.9966831
GATATAC1306.502954E-427.8678233
TACTACC1050.007728868627.6024134
TAGCATG6250.026.66612
TAGTACC2808.236384E-925.8772624
CTATATA2601.1445081E-725.093281
CTATTAT2351.3303707E-624.6780261
ACTATAC1801.5255787E-424.1521133
TTAGAGT2401.6048052E-624.1521114
CTAGGGC1851.8370355E-423.4993533
TTATACT3402.5174813E-923.4417574
GTATAGC1550.001808903423.384421
CCCCGGA1550.00181490523.3711139
TACTGAA8150.023.1149047
Produced by FastQC (version 0.11.5)