FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1910-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1910-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779550
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA300.001939042672.467357
TCGTATG450.00964816248.3208732
ATAGCGT450.00965550848.3115658
TATAACC2350.037.00463
TATACTG4400.034.582255
GTTACGT900.003631181832.2159841
CTAGTAC900.003635753232.207713
ACGAATT900.003638040532.2035756
GTATATA2451.9736035E-929.586111
CGTATGT1000.006087023828.9869383
TAGGACT4400.027.9987494
GTCTAAC1050.00771470527.61371
TACTGAA9050.027.2253027
CTATACT1606.847194E-527.1752574
CTACTGA8150.025.7826166
TATAGAG2051.2955867E-524.741775
GTCCTAT8050.024.3120631
GTTATGT4700.023.133821
AGCGAAA4103.8198777E-1122.9833551
GCTACTA1600.002180421722.6460469
Produced by FastQC (version 0.11.5)