FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1910-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1910-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185138
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT27370.23094356944085834No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA16430.13863364435196576No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC13310.11230759624617556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1200.084.561811
GTTACCG604.9341406E-448.355778145
AGCGAAA3800.036.2407721
ACCGTGT12750.035.8007978
GCACCGT13150.035.2583126
AGCACCG13900.034.917975
ACGTGTT3000.033.8090064
CCGTGTT13900.033.360119
GTGATCG1153.174242E-431.5017389
TAGCACC16400.031.3646264
CACCGTG15200.030.9836167
CGTGTTG3450.029.3966525
CCGACTA2252.8378054E-828.9791494
GCGTGCG3650.027.7905719
GCGAAAG4750.027.4620512
ACATAGC17300.027.2329521
GTTATGT6500.026.762421
CATAGCA18750.026.6687032
TCGATTA4250.025.600117145
AGACGAC2559.551695E-825.5709173
Produced by FastQC (version 0.11.5)