FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12845987
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1419171.1047574623888379No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATG212380.16532789578566442TruSeq Adapter, Index 13 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG170700.13288196539510744TruSeq Adapter, Index 4 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC959400.043.99869
GATCGGA997900.042.392852
CGGAAGA1004850.042.2700235
AGATCGG1006150.042.196551
TCGGAAG1003950.042.141494
ATCGGAA1006200.042.0762983
AAGAGCA1097650.038.655278
GAAGAGC1136300.037.3324747
GGAAGAG1178800.036.236086
AATCGGA23300.023.7739521
GGATAGC41700.023.6920935
GCAGGTA40400.023.0176722
GACAGTA46750.022.9368087
TATACTG60250.022.5468125
CGAAAGC32550.022.5409723
ACTGATC50900.021.0676178
CAGGTAC46250.020.4189723
TTGGACA58300.020.3104554
CGATCTA17100.020.0329781
GGACAGT68300.018.9322956
Produced by FastQC (version 0.11.5)