FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_Negative_Control_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_Negative_Control_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599738
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1056.900336E-848.333319
GTATAAG2151.2732926E-1137.0960921
AGCGAAA2954.820322E-1027.0361351
GCGAAAG3255.0931703E-1126.7692172
TAGTACT2351.3289609E-624.6808384
TATACTG3601.8371793E-1024.1666535
GAGTGAT2451.9099662E-623.6734547
GTATAGG1550.001806777623.3890341
CGAAAGC3854.274625E-1022.5973913
GTGTAAG3106.2304207E-721.050131
TACAGGA3551.0130316E-720.4225232
AGGTCGG1800.004305109420.1388768
ATTAGGT2959.5518135E-619.6610053
GTACTGT2256.8594643E-419.3349341
TAGGACG1950.00683746218.5897335
CTATACT2359.1942545E-418.5106284
CATATTC4354.262074E-818.3333233
TAGTATG4354.262074E-818.3333232
GTATTGA2000.00790870218.1265031
GTACAGG3201.9244671E-518.1265011
Produced by FastQC (version 0.11.5)