FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_Neg-Control-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_Neg-Control-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122672
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4750.036.6331561
GGTACTG6100.033.2786375
GCGAAAG5500.032.9544942
CGAAAGC5600.032.366023
TGCACCG1352.142457E-532.2221765
GTACTGA6600.029.6590466
AGGTACT7450.029.1945864
ACTGATC8450.028.3135668
AGGTCGG5350.027.102768
CAGGTAC7000.026.928533
GGATAGC4600.026.7934365
GTTGACA9800.026.6337991
GGCCGAT1100.00967215426.3635967
GCAGGTA7850.025.859832
CGACACT2701.6492777E-724.166634
GCACCGA1500.001494400324.166636
TCGACAC2701.6492777E-724.166633
TATACTG7900.022.9430035
GTTATGT7600.022.8957211
GTATGGA7000.022.7866951
Produced by FastQC (version 0.11.5)