FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_HK_1073_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_HK_1073_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAAAGAAAAAAAGTAAAAAAAAAAAATCAAATGCAATGAAGAAAAA18.333333333333332No Hit
TTACTAGACGGTTGTACCGCGAGCGCCCCCTGATACGGCTGCTGCATCAT18.333333333333332No Hit
GATGTTTTATCATTAATGCATTAAGAAGCTGAACAAGCAAGAAGACCAAA18.333333333333332No Hit
AGTCATTAGCACACAGAGGATGTGCTAATATGAGAAAAAATGGACTTTCT18.333333333333332No Hit
GTTTCTGAAGTAATGGTACCAGTTCCTCCTTGTACCTCTGGTAGAATTCG18.333333333333332No Hit
TTCCAGGACCTCCGCAAGCCCTGCTCTGGAAGTCAGGAGACCCCCCTGCC18.333333333333332No Hit
GGACATACTTATACTAAAACATTCGTTGTTTATCTCAAATTCAAGTTGAA18.333333333333332No Hit
AATCAGCTCCTTGCAAACAAAAAAACCACAGCTACCAACGGTGGAATGTT18.333333333333332No Hit
ACAGAGGCTTAACCCACTGAGCCACCCAGATGCCCTCAGTTCTGTTTTAT18.333333333333332No Hit
ATCATAGGGAACTTTAATACCTCACACTTCCCTCAATGATCCCCCATCCA18.333333333333332No Hit
AACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATACTGACAAAA18.333333333333332No Hit
TAATTCAGAGTCAGTGTGGCTGTGTTTTAATCACTTCTGATTTGAGGAGT18.333333333333332No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)