FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1987_d06_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1987_d06_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences582118
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTATCCTATGGGGAATTAATGGAAGATCCAGAACTAGAGCCTAGGTT8840.15185924503279402No Hit
GCCTAAGGGAGGATGACCAAGAAGCATGGGGCTGCGGTCTTTGGTATCAA8400.14430064007641064No Hit
ATCTAGTCCCTATCCCTGAACTCAGCCCAGAGCCGCCTTCTGCACATCTG8380.14395706712384773No Hit
CAATATACAGATTACCTTTTTTTAAACTGGAGTTACCCAACAAGTGGACC7920.13605488921490144No Hit
TCTATGGGCAGTGTATTTTTTGCTACCCCAAGCTGCCTGCAGGCTTTCTG7540.12952700311620666No Hit
TCACTAGACAGTGGCTATGGAGAAAAGTAGATGGAATCAAAAAATACTGA7520.1291834301636438No Hit
TGGTAGAGCAGGTGTTGAGGCACAAGGACACCAGCCAAGGGAGCAAACGA7260.12471698178032632No Hit
GGATAATGGGTTGTGCCAGGTAACCAAAGCAGAATCCGTGATGGAAAATC5830.10015151567208022No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1200.0102.708091
TATCCTA1950.078.0767445
GGGAACG350.00356578962.1427089
GACGGGT502.0095665E-457.9998637
GCGAAAG2150.057.3254472
AGACGCG400.006047621454.379543145
GCGTTTA400.00604967754.374878
CTATCCT3500.047.6427424
TCGCCAT1109.943142E-846.140217145
TATACTG1307.3414412E-944.6152845
ATCCTAT3350.043.283486
CGAAAGC3450.039.927443
CCTATGG4050.037.5925038
AAGTAGT800.002035940836.2499166
GCGTCCG800.002035940836.2499169
TTATACC3450.035.7245524
GCCGGTG850.002741079834.117577
ACGGGTA850.002741079834.117578
AAGGGTG2353.45608E-1133.936095
GAATAAC1501.132832E-633.8332521
Produced by FastQC (version 0.11.5)