FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1987_d04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1987_d04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940195
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCAGACTGCTCAGGGTATAGCCCAAGGAACGGGGCTAAAGATAGAAG19190.204106594908503No Hit
ACTTATTACCGTCTTCAGAAAATCCAAGATATGGAAGGTGGAAGAAAGAA13500.14358723456304276No Hit
GTATATACTCAATAGGTAAGCTGAATGCTTACCTGCTTTATAAAGTCTGT11260.11976238971702678No Hit
GTCACACACCCTGTGGGAATAAGTCACAGTTTCCCAAGTGTTCACTACAG10090.10731816272156308No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2750.092.28231
CGAAAGC3900.066.926463
GCGAAAG4450.057.0253522
TTACCGT2950.054.0705346
ATTACCG3300.048.3357775
CCGTCTT3650.043.6985179
TACCGTC4000.043.4998867
ACCGTCT3900.040.897338
TATTACC4250.037.5313154
ACGTATC800.002036579236.2499057
CCCTATA3300.035.1532942
ATATGCC3450.033.6231048
TATATAG2008.94579E-932.6266485
CTATAGA4550.031.871441
GTCCTAT7400.031.3546091
GAGCGAA950.00472475430.5294841
GATATAG2401.5807018E-930.2082589
CGTATCC1453.490236E-529.9999248
CTATATA2252.8152499E-829.0014694
CCTATAT2252.8152499E-829.0014693
Produced by FastQC (version 0.11.5)