FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1987_d02_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1987_d02_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959865
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC15370.16012668448167192No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12520.13043500908981992No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT9730.10136842160095431No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1652.7284841E-1143.9415441
TAGTCGG1157.1425675E-637.8259628
GTATATA4750.032.05421
TTACGTG2401.5807018E-930.209812
GTGATCG1358.0826273E-426.8517659
CTATACA2756.7648216E-926.3635524
GGTTATA3451.07320375E-1025.2186241
GTAGCAT15950.025.001221
TATACTG4650.024.9461545
GCGAAAG3753.074092E-1023.2011342
CTACTGA10400.023.0047346
GTTATGT4800.022.6573581
CGTGTTG3203.3853212E-822.6561785
TAGCATG14300.022.3087832
GTAATAG2303.1406686E-522.0662961
GTCTAAC1650.002600046621.9707721
TACGTGT3304.6895366E-821.9696253
AGCGAAA4006.89397E-1021.7510621
ATATAGG2353.7090722E-521.5956766
TACTGAA11450.021.5283157
Produced by FastQC (version 0.11.5)