FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1987-d12-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1987-d12-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050972
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA553.219312E-452.7264941
TATCGTC450.00963621248.337227
TACCGAC700.00105624841.43192
ACGGTAC800.00203585936.2529143
TATACTG3250.033.464235
ATGCTCG950.004727744530.5258669
AGCGAAA4600.028.3691481
TTACGCT1100.00966801426.3657554
GCGAAAG5200.025.098172
AACCGCG1851.8297555E-423.5154047
CGAAAGC6000.022.9601783
GTCCTAT9300.020.2685181
GTACTGG3701.5702426E-719.5943051
GTTATGT6300.019.5632041
GTGTAAG4853.601599E-1019.4327031
TCTATAC8250.019.3348883
CCGTAGA2256.8686804E-419.3330481
GTATATC2256.8686804E-419.3330481
TCGATTA4954.7475623E-1019.040121145
TAATAAC3051.2741619E-519.0179213
Produced by FastQC (version 0.11.5)