FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1987-d10-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1987-d10-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321789
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT13920.10531181603115171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4650.032.749231
GCGAAAG5200.029.2809372
GTTACGT1804.69332E-628.200731
ATAACGC1100.00966932126.365523
TTAGGTA5700.025.4404144
GTACGGA1450.001226141225.0017856
GTATTAG6400.023.7943631
TCGATTA5500.023.727173145
CGAAAGC6600.023.0698323
TACGCAG1650.002600218421.971272
GGATTAG6400.021.5282331
GGTAGCG3757.768904E-921.2665778
GTCTAGG6200.021.0530781
TAGTGCA4851.6370905E-1120.9277489
ATTAGGT7300.020.8576553
GATTAGG5950.020.7157672
GTAGCGG4001.6329068E-819.9374169
TAGGTAG8850.019.6624225
GCACCGT12850.019.184256
GTTGACA10050.018.7603971
Produced by FastQC (version 0.11.5)