FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1987-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1987-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888114
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG451.1938333E-464.443925
TACCCCG750.00148335638.6663555
GTTACGT1209.559488E-636.2517471
TCGGCGT900.003628159432.221964
GATATCG1255.1536266E-428.9997639
TAGTCGG1255.1536266E-428.9997638
GTTATGT6350.028.5446831
ATACCGA1100.009671397526.3634226
ATTATAC2859.893483E-925.4383893
TATACTG4001.8189894E-1225.3747925
GTATAAT2901.1896191E-825.0012051
TTACGTG1751.2556407E-424.856942
GCGAAAG3801.2732926E-1124.8038251
GTTGACA7450.024.330031
ACATGGT8200.022.9876165
GTAATAC2852.778288E-722.8945483
GCACCGT5001.8189894E-1221.7498236
CGAAAGC4006.89397E-1021.749822
TAGGGTG3706.6484063E-921.5538795
CTATATA3406.426126E-821.3245561
Produced by FastQC (version 0.11.5)