FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1987-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1987-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295328
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG701.6930513E-551.785711
GTATAGG901.3203808E-648.3333321
TATAGGG951.916258E-645.7894742
GAGCGAA657.328155E-444.6153831
GTATTGA1105.251699E-639.5454521
TGATAGT800.00203429636.254
GTCTAAT1001.389076E-436.251
GTCTATG900.003624123832.222221
ATTAGAC950.004721960530.5263183
AGCGAAA1204.0511924E-430.2083341
GGGTTGA1703.002251E-629.8529426
GCGAAAG1306.472053E-427.8846152
CTATGAC1050.00769980227.6190473
GGCAACG1050.00769980227.6190477
TGTAGTC1050.00769980227.6190473
GTATGGA1855.797958E-627.4324341
CGAAAGC1658.398865E-526.3636363
GTGTATA1658.398865E-526.3636361
ACTGCTA1409.977876E-425.8928558
TTGAAAC2202.2219272E-523.068189