FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1987-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1987-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592635
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGGACTGTGCAGTTTAAGGCAGTTAACTCTACCTTGAAACCCAAAG8010.13515907767850363No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGGT100.0070867594145.023122
AGCGAAA1550.0112.275961
GCGAAAG2300.088.274952
CGAAAGC2600.078.089373
GTATAGG754.843969E-967.677461
GAGCGAA350.003563535262.1527671
GGCGAAA907.47172E-540.28421
CCGTCTA750.001482966138.6663069
GGACGGT1157.134113E-637.8289226
GGAGGAC4400.034.607793
TATAGGC1102.4326795E-432.95982
GTACAGG2601.10958354E-1030.6779691
TATACAG1453.4862336E-530.002255
TTATAGT1952.6025555E-729.7458214
CGGTGAA1000.006072764328.9997319
CAGGACG2603.7107384E-927.8867074
CTATAGC1855.803695E-627.4368083
AGCTATC1907.149516E-626.7102787
TAGTGGA2559.458017E-825.5923162
TCGGACT1701.0300727E-425.5901554
Produced by FastQC (version 0.11.5)