FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1986-d10-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1986-d10-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825534
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC25840.3130095186872982No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC24800.3004116123624224No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG19690.2385122841700039No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG18320.2219169652612733No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA18170.2200999595413393No Hit
GGGTAATGGTATCACCTCTGGCTGTAACGTGGTGGAACAGAAATGGACCA15550.18836292629982535No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA14930.18085263599076476No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG14630.17721862455089676No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA14320.17346347939636647No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG13800.16716452623392858No Hit
ATCCAAAGGTGTATAAAACCTACTTTGAAAAGGTTGAAAGATTAAAACAC13550.1641361833673719No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA13320.16135010793013976No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT13300.16110784050081525No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT13170.15953310221020575No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC12750.15444548619439052No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA12590.15250734675979427No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC12240.14826766674661493No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT12230.14814653303195263No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC12230.14814653303195263No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT12120.14681406217066773No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT11940.1446336553067469No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT11790.1428166495868129No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT10780.13058214440592392No Hit
GTTCATTGATGCTTAATGCTGGTCCATATCTTTTGTCTTCTTTGCCGAGG10640.12888627240065217No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG9890.11980124380098216No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA9890.11980124380098216No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC9750.11810537179571041No Hit
GCTTAATGCTGGTCCATATCTTTTGTCTTCTTTGCCGAGGATTAGAAATC9610.11640949979043869No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC9480.1148347614998292No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT9400.11386569178253106No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG9210.111564151203948No Hit
GTCTAACAGCCAATGAGTCAGGAAGGTTAATAGATTTCCTCAAGGACGTA9200.11144301748928571No Hit
TAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGA9140.11071621520131211No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCATTCTGCTGT9010.10914147691070265No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA8950.10841467462272905No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG8910.10793013976407997No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC8860.10732447119076863No Hit
CCATAGAGGTATTCAGATCGAACGGTCTAACAGCCAATGAGTCAGGAAGG8810.10671880261745731No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC8740.10587086661482142No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG8690.1052651980415101No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG8630.1045383957535365No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT8470.10260025631894022No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT8470.10260025631894022No Hit
CTATAGGACTTGCAAGCTAGTTGGAATCAACATGAGCAAAAAGAAGTCTT8410.10187345403096662No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG8310.10066211688434396No Hit
CTATTGCTCCTTCATCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGT8260.10005644831103261No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGTC100.0070909234144.998063
GGTACTG1850.0101.8905265
CGAAAGC1850.0101.8905263
GCGAAAG1900.099.2212142
AGTAGAA3700.097.983531
TTCTACT502.2684617E-886.998854
GCAGGTA2400.081.571292
AGGTACT2650.071.131134
AAATATA1800.064.443585
GTCATAT1250.063.8068771
TAACTAT2400.063.4443361
AACACTG5200.058.556915
TTTCGTT651.0931706E-555.771866145
AGAAACA6550.055.3427734
CAAGGTA1850.054.8641329
CGAACTT400.00604938454.377567145
GTACTGA4200.053.5111926
GGTCAAA2200.052.7329561
TACTAAT4400.052.7329562
CTACTAA4500.051.561111
Produced by FastQC (version 0.11.5)