FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1986-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1986-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences763422
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG28580.37436699492548026No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC23050.30192999415788385No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT15860.20774879424486065No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG14220.1862665734023908No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA13470.17644238704150522No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA12620.1653083091658349No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC12360.16190259122739456No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT11320.1482797194736332No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC9690.12692848778264185No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG9460.12391573729863693No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT9440.12365375899567999No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG9220.12077199766315352No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT9140.11972408445132574No Hit
CCGCTATATGATGCGATAAAATGCATGAAAACATTCTTCGGCTGGAGAGA8250.10806604996974149No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT7920.10374340797095186No Hit
CTATAGGACTTGCAAGCTAGTTGGAATCAACATGAGCAAAAAGAAGTCTT7700.10086164663842541No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTG1200.084.5833365
GCAGGTA1550.065.483872
AGGTACT1700.063.970594
GGTTATA400.0060503454.3751
AGTAGAA3250.053.538461
AACACTG3550.051.056345
ATATCAC1004.70136E-850.753
GCGGTAT5200.048.798086
TATAGCG5200.048.798082
GCCCTAC450.009638268548.3333321
CGCAATT450.009638268548.333332145
GGATAGC3700.045.0675665
GCTTATA657.335487E-444.6153872
GTATCCC5750.044.1304369
CGGTATC5900.043.0084727
GTACTGA3150.041.4285746
GCGAAAG1053.82293E-641.428572
CTAATAG4450.040.730344
GGTATCC6250.040.6000028
CTATTAG10250.039.6097561
Produced by FastQC (version 0.11.5)