FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1986-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1986-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804832
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3000.045.9214861
GCGAAAG3800.038.1642722
CAATCCG1001.3913657E-436.2493029
CGAAAGC5000.030.4531963
ACTTACG1358.07931E-426.8530048
TCTATAC4950.024.9016063
ACTATAC1550.00180773323.3880988
TATACCA6350.022.8370445
GTCTAGG3004.5480374E-721.7522831
TATAAGG3406.416121E-821.3270932
TACACCG1700.003090393721.3257685
CTATACC5800.021.2522324
CCTATAG2806.082304E-620.7164613
CTATAGT2505.9678212E-520.302131
ATAGGAC3601.1740303E-720.1410053
GTATAGG1800.00430373620.1410051
ATAATAC5052.910383E-1120.101123
GTCCTAT7650.019.9040491
TAATGGT2556.949714E-519.9040494
ATACAAG3701.5671321E-719.5966536
Produced by FastQC (version 0.11.5)