FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1986-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1986-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACCAATGACTCGTGTGTGTATCCTTGCCTTGCAAATTCAGCTAACCTA12.4390243902439024No Hit
CCCCCACCATTGACACTTCACCATGGCACTGATACCAAGGAACGAAAGAA12.4390243902439024No Hit
GTCCCCTGGCTCCTGGCCACTGCCAGCCTACTTTCTGTCTCTATAGTTTT12.4390243902439024No Hit
TTTATACATATTCTCGTATTGGAGTCAGGATATGGTCATTGGAGAAGAAT12.4390243902439024No Hit
TTGTTGTAGTCCTGTACGCTGACTAATCTGCCCTTGAAAATCAGATAAAC12.4390243902439024No Hit
AGGCAGGAGGCATTTACTCTGAGCTTTAGGGGGCCCGAGCTCCCAAGGGT12.4390243902439024No Hit
CCTGGGACCGTACATGTCACTAACAGGCCATTATATACTAGGTTATAAAG12.4390243902439024No Hit
GAATGCTATGCTTTCACAGAGGTTTTCAGCGTGGAGCGAGCCGCACATCG12.4390243902439024No Hit
GGACAGGAGCTGCTGTGATTCTCGATTGGAAACTAATGATAAAAGACAGA12.4390243902439024No Hit
GAATCCTAAAGAAACAGACATCTGACTGTTCAGCTGTCTACTAATGACCA12.4390243902439024No Hit
CATACAAACTTACACAAAACACACAACCTTAACTAAAGATTATAAAACAA12.4390243902439024No Hit
GAACTAGATAGGGTGAGACGGAAAGGATGTTGAACCAGTTCTACCTGTAG12.4390243902439024No Hit
AAATGTATTTACCAATATTTGATCCTAAGCACCGACACCAGATAAAAATG12.4390243902439024No Hit
CACAAGAGAGGCAGGATACCACCCCAGAGCCCAATGATCTTTCTCAGAAT12.4390243902439024No Hit
CTATATATGTATTACTATATTAAATTTCCAACTTACTAAAAACTTTGAAA12.4390243902439024No Hit
AGGTTGGGGAAACCCTTGAAGTAGCCGTTCGGGATGGTCTCGATCCTGTT12.4390243902439024No Hit
CCAGTAGTGTGACCCCACTTTCCATAGCTGGAGAATGTATTCATCACTAT12.4390243902439024No Hit
GGTGGTAGGGTACAATTTACTTCACTCTACAACTTCCTTCTTCTTCTGAA12.4390243902439024No Hit
CTATGATATTGGTGCTGATTGCTTCTCTTAGGTTTGTTCATATATGCTCT12.4390243902439024No Hit
TATTAACTACGAGAGCCCCAACAATGGTGGTTGGCAGGGAGTCAGTGAAC12.4390243902439024No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT12.4390243902439024No Hit
CCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACAC12.4390243902439024No Hit
TCAGCATTCCGTCCGGGCACAGCCCATAGTAGGCACTCAGTAAGTGTGTG12.4390243902439024No Hit
ATTCTTCCCCTTAAAGCCTGAAATCTAGATAGCAGTAATTCATGGGGGAG12.4390243902439024No Hit
CCAAAACGAAATCATAAAAACAAAACACCTCAATGATAACTACAATAAAA12.4390243902439024No Hit
CCCTGGAATCATGACCTGAGCTGAAGGCAGATGCTTAACTGACTGAACCA12.4390243902439024No Hit
ATGTTTAGGTTCTAGAGGGAGACCAGGCAGTTGGAATTACTCTTGAAAAG12.4390243902439024No Hit
GCTTCCATTCTGTTAAATATCTTGAATGGCTCAGGAATGTTCAAGATATT12.4390243902439024No Hit
GAGTTCGAGACCAGCCTAGCCAACATGGTGAAACCCCATCTCTACTAAAA12.4390243902439024No Hit
GAATTGTGATGCCACCAACATTGGCTTTATTTAACAATATTTCTTTTGAT12.4390243902439024No Hit
CTACTAAGCTGTAAGCTTCATGAAAACAGGAACCAGATTTTGTATCCTGA12.4390243902439024No Hit
AATAGGAACACAGAAATACTTTGAGGTAAATGAAAAAATAATACAATGAA12.4390243902439024No Hit
TGTATGTGAAGCATTAAAAAACTCTTGTCGTTTTGGATTGTAAAACCAGT12.4390243902439024No Hit
CCCTGAAATACATCTTACTGGATCATACCTCTTCCCGTATTGTAGAATTT12.4390243902439024No Hit
CTCTACAGAGTATAGGCAATTTGTTTTCTGTTACTGGTTTTACAATCCAA12.4390243902439024No Hit
ATATATATATATGTATATATACATACATGCACACACACGTATGTATGTAT12.4390243902439024No Hit
GATCATGACCTGAGCCGAAGGCAGCTGCTCAACCAACTGAGCCACCCAGG12.4390243902439024No Hit
GAGTGGGAGAGGGGGAAGCAGGCTTCCCACTGAGCAGGGAGGCCGATGTG12.4390243902439024No Hit
GGTGGGGAAGCTTCCCCCACACAAAGAATTGATTTTAAGTGATGTGGAGA12.4390243902439024No Hit
AGGGAGAGAGGGTATCCTCCAGCAGCATCCCCGCTGAGTGTGGAGCCTGG12.4390243902439024No Hit
AATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTG12.4390243902439024No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)