FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1985_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1985_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718962
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAA553.2184008E-452.7272456
AGCGAAA2950.049.1559371
ATACGCC450.00963806148.333317
CGAAAGC4300.035.406963
GCGAAAG4250.034.1176342
TATAGAC1504.3970227E-528.9999873
TATACGA1000.00607335228.9999858
GTATATA3955.875336E-1022.0268361
ACTCGGC2003.101875E-421.7499896
GTCTAAA2353.7053396E-521.5972371
TCTATAC6100.021.3934333
GTATAGA3157.271083E-720.7157171
GTAGGGC1750.003657753620.7142773
TATACTG2155.054252E-420.2325485
CACGCGT1800.00430592520.138886
ACGCGTG1850.00504553819.5945847
GTACATA2608.069001E-519.5205781
GGATAGG4102.1624146E-819.4525641
GTGTATG4502.9849616E-919.334671
CTATACC7550.019.2066251
Produced by FastQC (version 0.11.5)