FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1985-d12-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1985-d12-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889072
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACA300.001935260472.503882
AGCGAAA3550.030.6371671
TATACTG4700.029.310085
GCGAAAG3950.027.533122
TGTAATA5400.026.8532892
GTATAAT3800.026.7134591
TACTGCG1100.00967114726.3635657
CGAAAGC4300.025.2920513
CTGATCG2101.5548485E-524.1666019
AGTTATC4103.8198777E-1122.9877438
TTATACT3503.4979166E-922.7869344
ATTATAC3554.120011E-922.4659923
GTCCTAT6250.022.042421
TACGGAG1700.003091568421.324675
GTATATA6150.021.2218421
ATAGTGC2806.086195E-620.7153956
TACACTA2908.243984E-620.001075
GTACCAC2556.9505026E-519.9041461
GTATAAC2608.069216E-519.5213741
GTGTAAT9200.018.915121
Produced by FastQC (version 0.11.5)