FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1985-d10-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1985-d10-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743222
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG8060.10844673596852622No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA7740.10414115836183536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG2200.046.142252
AGCGAAA2400.042.2999081
CGAAAGC2450.041.4310723
TTACGTG1252.7149508E-740.6051832
CCTATAC1604.584399E-836.252193
TATACTG3200.033.984145
GTTACGT1652.3817156E-630.7635731
GTTGACA6400.030.5918981
TTACCGC1504.3913635E-529.0056531
TACGTGT1907.1488466E-626.7121373
CTACATC3352.1063897E-923.8074073
CTACACC3052.0330845E-823.7703274
ACATGGT7650.023.6926465
ACTACAC1851.829302E-423.5149333
GACATGG8200.022.1035044
TAGTACT1700.003092180521.3233814
GTAGCGG2404.3611806E-521.1456859
GGTAGCG2404.3611806E-521.1456858
CTATACT2806.087168E-620.7141424
CCGACTA2108.712348E-620.7141424
Produced by FastQC (version 0.11.5)