FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1985-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1985-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847797
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCCG450.00963658948.335935
GTGATCG1303.6976417E-739.0382549
AGCGAAA3300.030.759231
GCGAAAG3550.028.5930842
GTTACGT2559.4776624E-825.589611
TTACGTG2559.4776624E-825.589612
TACGTGT2651.3751014E-724.6239643
GTATTAG2701.6474041E-724.1679651
CGAAAGC4455.456968E-1222.8102153
GTATAAC2755.2067007E-621.0920411
GTAATAT4501.3278623E-1020.945571
TATGCGG1750.0036571420.7153975
CTATGCG1800.00430520120.139974
GTTAGAC1800.00430520120.139973
TATACTG4702.3646862E-1020.0542665
GTATACC1850.005044688519.5956463
GTTGACA7350.018.7425041
CGGAGTA1950.00683759718.5907424
TCGCCAT1950.006839925418.589647145
GTAGCAT13650.018.0595781
Produced by FastQC (version 0.11.5)