FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1985-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1985-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011670
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC16470.16280012256961263No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG14140.13976889697233288No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA13700.13541965265353326No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC13070.12919232556070656No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG11440.11308035228878982No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA11430.11298150582699892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT2200.049.433991
TTACGTG2250.048.3354572
GTCTACG750.001483073738.6683651
GTTGACA9700.038.120231
TATACTG5700.033.0699965
TACGTGT3350.032.462513
TAGTCGG1801.2924829E-732.222058
AGGTCGG5400.032.2220468
ACATGGT11850.031.8141735
AGCGAAA4850.031.3931311
GTAGCAT14550.030.3965261
TGTTGGC15300.028.9050717
CAGGTCG6050.028.7601747
CGTGTTG4050.028.6418195
GTTATGT8700.028.334581
GTGTTGG16100.027.9191046
ACGTGTT3900.027.8844644
GCATGTT11800.027.6481554
TGACATG12900.027.5386093
GACATGG13250.027.3583414
Produced by FastQC (version 0.11.5)