FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1985-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1985-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771912
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAC900.00362674632.2239954
ACTCCGC1907.153212E-626.7102628
GAACGTC3357.4578566E-1125.969894145
GCGAAAG4200.025.894282
GACTGCG1409.993095E-425.8926017
AGCGAAA4350.025.0046121
GGATCGT1550.001807539423.3883826
GTATAAC3753.074092E-1023.2042831
CGAAAGC5000.023.2012753
GTCCTAT5650.023.1016061
CTGCGTA1600.002174828222.6560279
TCTCGAA1600.002174828222.6560278
GTATATA8350.021.7105921
GTACAGG3757.7398E-921.2705921
AGGCGTG1750.003656821320.7154245
GAATAAC4301.6934791E-920.2362921
ACGCTAA1800.004301598320.1426051
CGAAGGT1800.004304826320.1399964
GTATATG2908.232348E-620.003691
TATAGAG2908.241572E-620.0010995
Produced by FastQC (version 0.11.5)